Mikroorganismu bioenerģētikas laboratorija

• Baktēriju bioenerģētika, fizioloģija un respiratorā katabolisma metaboliska inženierija.
• Fizioloģija, elektronu transporta ķēde un aerobā metabolisma regulācija etanolu producējošajā baktērijā Zymomonas mobilis, tās centrālā metabolisma modelēšana.
• Omega taukskābes uzkrājošo dinoflagellātu metabolisms un sintrofā kopkultivēšana.
• Antimikrobiālie peptīdi (AMP), to darbības mehānismi un saistība starp baktēriju AMP rezistenci un to enerģētisko metabolismu.
   

Laboratorijas vadītājs

Vecākais pētnieks

Uldis Kalnenieks

R. Rutkis, Z. Lasa, M. Rubina, I. Strazdina, and U. Kalnenieks (2024) Eminent Antimicrobial Peptide Resistance in Zymomonas mobilis: A Novel Advantage of Intrinsically Uncoupled Energetics. Antibiotics,13,451. doi.org 10.3390/antibiotics13050451I. Strazdina, 

M. Bikerniece, E.R. Paegle, K. Shvirksts, M. Grube, Z. Lasa, R. Rutkis, and U. Kalnenieks (2023) Oxidative Phosphorylation for Aerobic Survival, but Not for Growth: The Peculiar ‘Make-Accumulate-Consume’ Strategy in Zymomonas mobilis. Fermentation 9: 951. doi.org/10.3390/ fermentation9110951

K. Berzins, R. Muiznieks, M.R. Baumanis, I. Strazdina, K. Shvirksts, S. Prikule, V. Galvanauskas, D. Pleissner, A. Pentjuss, M. Grube, U. Kalnenieks, and E. Stalidzans (2022) Kinetic and Stoichiometric Modeling-Based Analysis of Docosahexaenoic Acid (DHA) Production Potential by Crypthecodinium cohnii from Glycerol, Glucose and Ethanol. Marine Drugs 20:115. doi.org 10.3390/md20020115

K. Kovtuna, I. Strazdina, M. Bikerniece, N. Galinina, R. Rutkis, J. Martynova and U. Kalnenieks (2022) Improved hydrogen peroxide stress resistance of Zymomonas mobilis NADH dehydrogenase (ndh) and alcohol dehydrogenase (adhB) mutants. Fermentation 8:289.

R. Rutkis, Z. Ļaša, M. Rubina. R. Ščerbaka, G. Kalniņš, J. Bogans and U. Kalnenieks (2022) Antimicrobial activity of Zymomonas mobilis is related to its aerobic catabolism and acid resistance. Fermentation 8:77.

I. Strazdina, L. Klavins, N. Galinina, K. Shvirksts, M. Grube, E. Stalidzans and U. Kalnenieks (2021) Syntrophy of Crypthecodinium cohnii and immobilized Zymomonas mobilis for docosahexaenoic acid production from sucrose-containing substrates. Journal of Biotechnology 338:63-70.

U. Kalnenieks, K.M. Pappas, K. Bettenbrock (2020) Zymomonas mobilis metabolism: novel tools and targets for its rational engineering. Advances in Microbial Physiology 77:37-88.

U. Kalnenieks, E. Balodite, and R. Rutkis (2019) Metabolic Engineering of Bacterial Respiration: High vs. Low P/O and the Case of Zymomonas mobilis. Frontiers in Bioengineering and Biotechnology 7:327.

U. Kalnenieks, E. Balodite, S. Strähler, I. Strazdina, J. Rex, A. Pentjuss, K. Fuchino, P. Bruheim, R. Rutkis, K.M. Pappas, R.K. Poole, O. Sawodny and K. Bettenbrock (2019). Improvement of acetaldehyde production in Zymomonas mobilis by engineering of its aerobic metabolism. Frontiers in Microbiology 10:2533.

Balodite E., Strazdina I., Martynova J., Galinina N., Rutkis R., Lasa Z., and Kalnenieks U. (2019) Translocation of Zymomonas mobilis pyruvate decarboxylase to periplasmic compartment for production of acetaldehyde outside the cytosol. MicrobiologyOpen, DOI: 10.1002/mbo3.809

I. Strazdina, E. Balodite, Z. Lasa, R. Rutkis, N. Galinina and U. Kalnenieks (2018) Aerobic catabolism and respiratory lactate bypass in Ndh-negative Zymomonas mobilis. Metabolic Engineering Communications 7: e00081

Projekti finansēti no Latvijas zinātņu padomes:

• Oxygen-resistant and thermotolerant Zymomonas mobilis respiratory mutants (2018-2020);
• ERA-IB-15-109 ‘Z-Fuels’ (A novel bacterial system with integrated micro-bubble distillation for the production of acetaldehyde. Latvian national funding for the ERA IB 6th call (2016-2010);
• NADPH-dependent aerobic energetics of Z. mobilis (01.0401);
• Alternative mechanisms of cyanide-resistance in bacteria (04.1101);
• Physiological role of energetically uncoupled respiration in bacteria: the case of Zymomonas mobilis (09.1306);
• Metabolic engineering of Zymomonas mobilis respiratory chain (536/2012).

Projekti kas finansēti no ERAF un ESF: 

• Novel anti-microbial peptides from Zymomonas mobilis for treatment of skin and soft tissue infections. ERDF (2020-2023);
• Crypthecodinium cohnii and Zymomonas mobilis syntrophy for production of omega 3 fatty acid from byproducts of biofuel and sugar industry. ERDF (2019-2022);
• Translocation of acetaldehyde synthesis reaction from Zymomonas mobilis cell interior to its periplasm. ERDF. (2017-2020);
• Construction and study of biohydrogen-producing Escherichia coli strains In the scope of ERDF project 2010//0298/2DP/2.1.1.1.0/10/APIA/VIAA/157, Coordinated by Riga Technical University;
• Aerobic metabolic engineering of Zymomonas mobilis In the scope of ESF project 2009//0207/1DP/1.1.1.2.0/09/APIA/VIAA/128 Coordinated by Latvia University of Agriculture .

Early studies on Zymomonas mobilis aerobic energy metabolism during mid-nineties were done in collaboration with Prof. H. Sahm and Dr. S. Bringer-Meyer, Institute of Biotechnology-1, Research Centre of Juelich, Germany. Part of the recent research work on respiratory chain has been carried out at Prof. R. K. Poole's laboratory, University of Sheffield, UK. This cooperation has been supported by a NATO / Royal Society fellowship and by several Royal Society and BBSRC fellowships for short visits of Professor Kalnenieks to the University of Sheffield.

Agrīnie pētījumi par Zymomonas mobilis aerobās enerģētikas metabolismu deviņdesmito gadu vidū tika veikti sadarbībā ar prof. H. Sahm un Dr. S. Bringer-Meyer, Biotehnoloģijas institūtā-1, Jielich Pētniecības centrā, Vācijā. Daļa no nesenajiem pētījumiem par respiratoro ķēdi tika veikta prof. R. K. Poole laboratorijā Šefīldas Universitātē, Apvienotajā Karalistē. Šo sadarbību atbalstīja NATO / Karaliskās biedrības stipendija, kā arī vairākas Karaliskās biedrības un BBSRC stipendijas prof. Kalnenieka īslaicīgām vizītēm Šefīldas Universitātē.

Laboratorijas vadītājs

Dr.biol. Uldis Kalnenieks

Biotehnoloģijas Profesors

kalnen@lanet.lv

Telefons: +371 6703488

Fakss: +371 67034885