Latvijas Mikroorganismu kultūru kolekcija

Latvijas Mikroorganismu kultūru kolekcija (LMKK) ir Latvijas Universitātes Medicīnas un dzīvības zinātņu fakultātes Mikrobioloģijas un biotehnoloģijas institūta struktūrvienība. MSCL ir lielākā mikrobioloģisko resursu krātuve Latvijā. Tās darbība ir vērsta uz bioloģiskās daudzveidības saglabāšanu ex situ, kā arī uz mikrobioloģijas un biotehnoloģijas jomu attīstību, kas Latvijai ir tradicionāli raksturīgas un perspektīvas.

Kolekcijā glabājas vairāk nekā 1 700 kultūru – baktērijas, raugi un micēlijsēnes. Lielākā daļa mikroorganismu ir izolēti Latvijā un ir unikāli paraugi, bieži vien vienīgie sava veida eksemplāri. Visi mikroorganismi tiek uzglabāti metaboliski neaktīvā stāvoklī šķidrajā slāpeklī, nodrošinot to ilgtermiņa dzīvotspēju un ģenētisko stabilitāti.

LMKK kolekcijas mikroorganismi tiek izmantoti pētniecības un izglītības vajadzībām Latvijas Universitātē un citās akadēmiskajās institūcijās, kā arī komerciāliem pasūtījumiem. LMKK aktīvi iesaistās Latvijas Universitātes studiju procesā – katru gadu vairāki studenti izstrādā bakalaura un maģistra darbus, izmantojot kolekcijas materiālus.

LMKK ir Pasaules kultūru kolekciju federācijas (WFCC), Eiropas kultūru kolekciju organizācijas (ECCO), Pasaules mikroorganismu datu centra (WDCM) un Eiropas Mikroorganismu resursu pētniecības infrastruktūru konsorcija (MIRRI-ERIC) dalībniece.

Turklāt LMKK ir MIRRI-ERIC Latvijas nacionālā mezgla koordinatore. Kā starptautiskā depozitārija iestāde saskaņā ar Budapeštas līgumu, LMKK pieņem baktēriju, sēņu (tostarp raugu) un plazmīdu patentēšanas vajadzībām, izņemot patogēnos mikroorganismus, kas pieder 3. un 4. bioloģiskās bīstamības grupai.

Issuance of microorganism cultures:
EUR 28.46 + VAT per culture;

Deposition

• For public access – free of charge;

• For patenting - EUR 426.86 + VAT per culture;

• Confidential storage of microorganisms, where the depositor retains all ownership rights (safe-deposit);
  EUR 35.57 + VAT per culture per year;
   

Isolation, purification, and characterization of pure microorganism cultures;
Consultations for clients.

Lab lead

Asoc. Prof. Vizma Nikolajeva

vizma.nikolajeva@lu.lv

Coppola A., Nikolajeva V., Lauritano C. 2025. Evaluation of the unexplored antimicrobial activity against human pathogens of two PUAs and Pheophorbide a from marine microalgae. Applied Sciences, 2025, 15, 13221. https://doi.org/10.3390/app152413221

Seņkovs M., Dubova L., Alsiņa I., Krīgere I., Lazdiņa D., Vendiņa V., Marcinkeviča S., Nikolajeva V. Composition of organic fertilizers containing microorganisms and their effect on soil microbiological activity and plant growth. Applied Biosciences, 2025, 4, 57. https://doi.org/10.3390/applbiosci4040057

Janceva S., Svarta A., Nikolajeva V., Zaharova N., Rieksts G., Andersone A. Forest logging residue valorization into valuable products according to circular bioeconomy. Forests, 2025, 16, 1418. https://doi.org/10.3390/f16091418

Mezulis M., Arbidans L., Nikolajeva V., Lauberts M., Grinfelds U., Klavins M. Environmentally friendly extraction of bioactive compounds from spruce and pine needles to obtain extracts with antioxidant and antimicrobial activity. Environmental and Climate Technologies, 29, 184-200. https://doi.org/10.2478/rtuect-2025-0013 

Borska E., Kviesis J., Ramata-Stunda A., Nikolajeva V., Ansone-Bērtiņa L., Borodušķis M., Kļaviņš M. Bioactive lipids and allelopathic potential of the invasive plant Heracleum sosnowskyi: insights into its fatty acid composition, antimicrobial and cytotoxic effects. Frontiers in Pharmacology. 16, p.1-15. https://doi.org/10.3389/fphar.2025.1582694

Ņečajeva J., Borodušķe A., Nikolajeva V., Seņkovs M., Fiļipovičs M., Jakobija I. et al. Characterisation and dynamics of the fungal communities of Avena fatua and Echinochloa crus-galli seeds in soil seed bank. Weed Research, 64(4), 310–320. https://doi.org/10.1111/wre.12651

Janceva S., Andersone A., Zaharova N., Nikolajeva V., Svarta A.  Soil amendments based on forest logging residues on dill (Anethum graveolens l.) productivity and composition. Environment. Technology. Resources. Proceedings of the 15th International Scientific and Practical Conference. Volume 1, 177-181. https://doi.org/10.17770/etr2024vol1.7983

Janceva S., Andersone A., Lauberte L., Zaharova N., Nikolajeva V. Fruit shrubs twigs as a source of valuable oligomeric polyphenolix compounds with antibacterial and antifungal potential. Environment. Technology. Resources. Proceedings of the 15th International Scientific and Practical Conference. Volume 1, 173-176. https://doi.org/10.17770/etr2024vol1.7982

Andersone A., Janceva S., Zaharova N., Nikolajeva V., Jurkjane V. Aronia melanocarpa (black chokeberry) branches biomass as a source of valuable biologically active compounds with antioxidant and antimicrobial potential. Environment. Technology. Resources. Proceedings of the 15th International Scientific and Practical Conference. Volume 1, 34-38. https://doi.org/10.17770/etr2024vol1.7981

Zommere A., Klavins L., Nikolajeva V., Kukela A., Kaipanen K., Korpinen R., Klavins M. Extraction and investigation of the lipophilic fraction from Norway spruce (Picea abies) and Scots pine (Pinus sylvestris) forestry side-stream biomass. Wood Sci Technol 58, 1259–1284. https://doi.org/10.1007/s00226-024-01566-x

Bolmanis E., Grigs O., Elīna Didrihsone, Senkovs M., Nikolajeva V. Pilot-scale production of Bacillus subtilis MSCL 897 spore biomass and antifungal secondary metabolites in a low-cost medium. Biotechnol Lett 46, 355–371 . https://doi.org/10.1007/s10529-024-03481-4

Kurpnieks O., Senkovs M., Rimkus A. Challenges of SSF process for pea and wheat bran valorization using Trichoderma spp. for biocontrol agent prodution. Environment. Technology. Resources. Proceedings of the 14th International Scientific and Practical Conference. Volume 1, 93-100. https://doi.org/10.17770/etr2023vol1.7306

Pastare L., Žogota M., Kienkas L., Boroduskis M., Ramata-Stunda A., Reihmane D., Senkovs M., Skudrins G., Nakurte I. Exploring the Potential of Supercritical Fluid Extraction of Matricaria chamomilla White Ray Florets as a Source of Bioactive (Cosmetic) Ingredients. Antioxidants, 12(5), 1092; https://doi.org/10.3390/antiox12051092

Rimkus A., Namina A., Dzierkale M.T., Grigs O., Senkovs M., Larsson S. Impact of Growth Conditions on the Viability of Trichoderma asperellum during Storage. Microorganisms, 11(4), 1084; https://doi.org/10.3390/microorganisms11041084

Andersone A., Janceva S., Petersone L., Krasilnikova J, Zaharova N., Nikolajeva V., Rieksts G., Telysheva G. Lignocellulosic Waste Compounds for Pancreatic Lipase Inhibition: Preliminary Extraction by Freon, Obtaining of Proanthocyanidins and Testing on Lipase Activity. Metabolites, 13(8), 922; https://doi.org/10.3390/metabo13080922

Andersone A., Janceva S., Petersone L., Skadiņš I., Nikolajeva V., Logviss K., Zaharova N., Rieksts G., Telysheva G. A comparative analysis of the proanthocyanidins from fruit and non-fruit trees and shrubs of Northern Europe: Chemical characteristics and biological activity. Sustainable Chemistry and Pharmacy, 36, 101266; https://doi.org/10.1016/j.scp.2023.101266

Aunina K., Ramata-Stunda A., Kovrlija I., Tračuma E., Merijs-Meri R., Nikolajeva V., Loca D. Exploring the Interplay of Antimicrobial Properties and Cellular Response in Physically Crosslinked Hyaluronic Acid/ε-Polylysine Hydrogels. Polymers, 15(8), 1915; https://doi.org/10.3390/polym15081915

Borodušķe A., Ķibilds J., Fridmanis D, Gudra D., Ustinova M., Senkovs M., Nikolajeva V. Does peptide-nucleic acid (PNA) clamping of host plant DNA benefit ITS1 amplicon-based characterization of the fungal endophyte community? Fungal Ecology, 61, 101181 https://doi.org/10.1016/j.funeco.2022.101181

Nakurte I., Žogota M., Pastare L., Kienkas L., Senkovs M., Boroduskis M., Ramata-Stunda A. Valorization of Bioactive Compounds from By-Products of Matricaria recutita White Ray Florets. Plants, 12(2):396 DOI: 10.3390/plants12020396

Šleinus D., Sinka M., Korjakins A., Obuka V., Nikolajeva V., Brencis R., Savicka E. Properties of Sound Absorption Composite Materials Developed Using Flax Fiber, Sphagnum Moss, Vermiculite, and Sapropel. Materials, 16(3), 1060; https://doi.org/10.3390/ma16031060

Andersone A., Janceva S., Petersone L., Ramata-Stunda A., Nikolajeva V., Zaharova N., Rieksts G., Telysheva G. Anti-Inflammatory, Anti-Bacterial, and Anti-Fungal Activity of Oligomeric Proanthocyanidins and Extracts Obtained from Lignocellulosic Agricultural Waste. Molecules 2023, 28(2), 863; https://doi.org/10.3390/molecules28020863

Ņečajeva J., Borodušķe A., Nikolajeva V., Senkovs M. Epiphytic and Endophytic Fungi Colonizing Seeds of Two Poaceae Weed Species and Fusarium spp. Seed Degradation Potential In Vi tro. Microorganisms, 11(1), 184; https://doi.org/10.3390/microorganisms11010184

Ramkumar N., Plantus K., Ozola M., Mishnev A., Nikolajeva V., Senkovs M., Ošeka M, Veliks J. Photoredox-Catalyzed Direct C-H Monofluoromethylation of Heteroarenes. Diversity, 15(1), 8; https://doi.org/10.3390/d15010008

Microbial Strain Collection of Latvia

University of Latvia, Faculty of Medicine and Life sciences

Institute of Microbiology and Biotechnology

Jelgavas str., 1 Riga, LV-1004, Latvia

Telephone: +371-67033925

E-mail: collect@edu.lu.lv